A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
HIGH-FREQUENCY TRANSFER OF LINEAR DNA CONTAINING 5'-COVALENTLY LINKED TERMINAL PROTEINS - ELECTROPORATION OF BACTERIOPHAGE-PRD1 GENOME INTO ESCHERICHIA-COLI
Tekijät: LYRA C, SAVILAHTI H, BAMFORD DH
Kustantaja: SPRINGER VERLAG
Julkaisuvuosi: 1991
Lehti:: Molecular and General Genetics
Tietokannassa oleva lehden nimi: MOLECULAR & GENERAL GENETICS
Lehden akronyymi: MOL GEN GENET
Vuosikerta: 228
Numero: 1-2
Aloitussivu: 65
Lopetussivu: 69
Sivujen määrä: 5
ISSN: 0026-8925
DOI: https://doi.org/10.1007/BF00282449
Tiivistelmä
Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.
Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.
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