A1 Refereed original research article in a scientific journal
HIGH-FREQUENCY TRANSFER OF LINEAR DNA CONTAINING 5'-COVALENTLY LINKED TERMINAL PROTEINS - ELECTROPORATION OF BACTERIOPHAGE-PRD1 GENOME INTO ESCHERICHIA-COLI
Authors: LYRA C, SAVILAHTI H, BAMFORD DH
Publisher: SPRINGER VERLAG
Publication year: 1991
Journal:: Molecular and General Genetics
Journal name in source: MOLECULAR & GENERAL GENETICS
Journal acronym: MOL GEN GENET
Volume: 228
Issue: 1-2
First page : 65
Last page: 69
Number of pages: 5
ISSN: 0026-8925
DOI: https://doi.org/10.1007/BF00282449
Abstract
Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.
Using electroporation with the phage PRD1 genome, we set up a high-frequency DNA transfer system for a linear dsDNA molecule with 5'-covalently linked terminal proteins. The transfer was saturated when more than 100 ng of PRD1 genome was used. Electroporation efficiency was about four orders of magnitude higher than that obtained with transfection. Removal of the terminal protein abolished plaque formation, which could not be rescued by supplying the terminal protein or phage DNA polymerase or both in trans.
UTU Research Portal