Adherence of Streptococcus mutans to Fiber-Reinforced Filling Composite and Conventional Restorative Materials. - UTU Research Portal

A1 Refereed original research article in a scientific journal

Adherence of Streptococcus mutans to Fiber-Reinforced Filling Composite and Conventional Restorative Materials.

Authors: Lassila LV, Garoushi S, Tanner J, Vallittu PK, Söderling E.

Publication year: 2009

Journal: Open Dentistry Journal

Volume: 3

First page : 227

Last page: 232

DOI: https://doi.org/10.2174/1874210600903010227

Web address : https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817876/

Abstract

AbstractOBJECTIVES:

The
aim was to investigate the adhesion of Streptococcus mutans (S. mutans)
to a short glass fibers reinforced semi-IPN polymer matrix composite
resin. The effect of surface roughness on adhesion was also studied. For
comparison, different commercial restorative materials were also
evaluated.

MATERIALS AND METHODS:

Experimental composite
FC resin was prepared by mixing 22.5 wt% of short E-glass fibers, 22.5
wt% of IPN-resin and 55 wt% of silane treated silica fillers using high
speed mixing machine. Three direct composite resins (Z250, Grandio and
Nulite), resin-modified glass ionomers (Fuji II LC), amalgam (ANA 2000),
fiber-reinforced composite (FRC) (everStick and Ribbond), and
pre-fabricated ceramic filling insert (Cerana class 1) were tested in
this study. Enamel and dentin were used as controls. The specimens
(n=3/group) with or without saliva were incubated in a suspension of S.
mutans allowing initial adhesion to occur. For the enumeration of cells
on the disc surfaces as colony forming units (CFU) the vials with the
microbe samples were thoroughly Vortex-treated and after serial
dilutions grown anaerobically for 2 days at +37 degrees C on Mitis
salivarius agars (Difco) containing bacitracin. Bacterial adhesion was
also evaluated by using scanning electron microscopy. Surface roughness
(Ra) of the materials was also determined using a surface profilometer.
All results were statistically analyzed with one-way analysis of
variance (ANOVA).

RESULTS:

Composite FC resin and other
commercial restorative materials showed similar adhesion of S. mutans,
while adhesion to dentin and enamel was significantly higher
(p<0.05). Surface roughness had no effect on bacterial adhesion.
Saliva coating significantly decreased the adhesion for all materials
(p<0.05). Composite FC resin had a significantly higher Ra value than
control groups (p<0.05).

CONCLUSIONS:

Short
fiber-reinforced composite with semi-IPN polymer matrix revealed similar
S. mutans adhesion than commercial restorative materials.

KEYWORDS:

Bacterial adhesion; fiber composite resin; restorative materials.; surface roughness