Isotopic labelling of peptides in tissues enhances mass spectrometric profiling



: Santos HM, Kouvonen P, Capelo JL, Corthals GL

: 2012

 Rapid Communications in Mass Spectrometry

: RAPID COMMUNICATIONS IN MASS SPECTROMETRY

: RAPID COMMUN MASS SP

: 3

: 26

: 3

: 254

: 262

: 9

: 0951-4198

DOI: https://doi.org/10.1002/rcm.5325


CONCLUSIONS: For first time the feasibility of O-18-labelling of peptides in situ for tissues has been demonstrated. The method decouples protein digestion from peptide labelling and is performed in sequential steps. Furthermore, we observed that O-18 incorporation produces characteristic isotopic peptide distributions, thus making facile distinguishing peptides from other tissue molecular components that ionise in the MALDI ion source. Copyright (C) 2012 John Wiley & Sons, Ltd.



Last updated on 26/11/2024 07:41:06 PM