A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Isotopic labelling of peptides in tissues enhances mass spectrometric profiling
Tekijät: Santos HM, Kouvonen P, Capelo JL, Corthals GL
Kustantaja: WILEY-BLACKWELL
Julkaisuvuosi: 2012
Lehti: Rapid Communications in Mass Spectrometry
Tietokannassa oleva lehden nimi: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Lehden akronyymi: RAPID COMMUN MASS SP
Numero sarjassa: 3
Vuosikerta: 26
Numero: 3
Aloitussivu: 254
Lopetussivu: 262
Sivujen määrä: 9
ISSN: 0951-4198
DOI: https://doi.org/10.1002/rcm.5325
Tiivistelmä
CONCLUSIONS: For first time the feasibility of O-18-labelling of peptides in situ for tissues has been demonstrated. The method decouples protein digestion from peptide labelling and is performed in sequential steps. Furthermore, we observed that O-18 incorporation produces characteristic isotopic peptide distributions, thus making facile distinguishing peptides from other tissue molecular components that ionise in the MALDI ion source. Copyright (C) 2012 John Wiley & Sons, Ltd.
CONCLUSIONS: For first time the feasibility of O-18-labelling of peptides in situ for tissues has been demonstrated. The method decouples protein digestion from peptide labelling and is performed in sequential steps. Furthermore, we observed that O-18 incorporation produces characteristic isotopic peptide distributions, thus making facile distinguishing peptides from other tissue molecular components that ionise in the MALDI ion source. Copyright (C) 2012 John Wiley & Sons, Ltd.
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