A1 Refereed original research article in a scientific journal
Engineering of a broad-specificity antibody: Detection of eight fluoroquinolone antibiotics simultaneously
Authors: Leivo J, Chappuis C, Lamminmaki U, Lovgren T, Vehniainen M
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication year: 2011
Journal: Analytical Biochemistry
Journal name in source: ANALYTICAL BIOCHEMISTRY
Journal acronym: ANAL BIOCHEM
Number in series: 1
Volume: 409
Issue: 1
First page : 14
Last page: 21
Number of pages: 8
ISSN: 0003-2697
DOI: https://doi.org/10.1016/j.ab.2010.09.041(external)
Abstract
Recombinant sarafloxacin-recognizing antibody was engineered with the use of novel fluoroquinolone (FQ) derivatives. A monoclonal FQ antibody, 6H7, was targeted to random mutagenesis to broaden the specificity of the antibody in development of a generic assay for FQ antibiotics. Engineering involved the synthesis of different small-sized FQ molecules to immobilize and detect the mutant antibodies. Selections with labeled FQs resulted in several mutant antibodies with increased affinity or wider specificity toward different FQs. The best characterized mutant antibody was capable of recognizing seven of eight targeted FQs below maximum residue limits set by the European Union. The results are promising in regard to the development of a multiresidue immunoassay for FQs based on a single antibody. (C) 2010 Elsevier Inc. All rights reserved.
Recombinant sarafloxacin-recognizing antibody was engineered with the use of novel fluoroquinolone (FQ) derivatives. A monoclonal FQ antibody, 6H7, was targeted to random mutagenesis to broaden the specificity of the antibody in development of a generic assay for FQ antibiotics. Engineering involved the synthesis of different small-sized FQ molecules to immobilize and detect the mutant antibodies. Selections with labeled FQs resulted in several mutant antibodies with increased affinity or wider specificity toward different FQs. The best characterized mutant antibody was capable of recognizing seven of eight targeted FQs below maximum residue limits set by the European Union. The results are promising in regard to the development of a multiresidue immunoassay for FQs based on a single antibody. (C) 2010 Elsevier Inc. All rights reserved.
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