Detection of circulating Yersinia-immunoglobulin complexes by enzyme immunoassay (EIA).

: Lahesmaa-Rantala R, Granfors K, Toivanen A

: 1986

: Journal of Immunological Methods

: Journal of immunological methods

: J Immunol Methods

: 89

: 2

: 191

: 9

: 0022-1759

DOI: https://doi.org/10.1016/0022-1759(86)90357-1

An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.