A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Detection of circulating Yersinia-immunoglobulin complexes by enzyme immunoassay (EIA).
Tekijät: Lahesmaa-Rantala R, Granfors K, Toivanen A
Julkaisuvuosi: 1986
Journal: Journal of Immunological Methods
Tietokannassa oleva lehden nimi: Journal of immunological methods
Lehden akronyymi: J Immunol Methods
Vuosikerta: 89
Numero: 2
Aloitussivu: 191
Lopetussivu: 9
Sivujen määrä: 9
ISSN: 0022-1759
DOI: https://doi.org/10.1016/0022-1759(86)90357-1
Tiivistelmä
An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.
An enzyme immunoassay (EIA) was developed for the detection of Yersinia-immunoglobulin complexes of known Ig class. Immune complexes (ICs) were attached to polystyrene microtiter plates by rabbit anti-human immunoglobulins, and the existence of Yersinia enterocolitica O:3 antigens was demonstrated using Fab fragments of alkaline phosphatase (AP)-conjugated antibody against the same serotype. Simultaneous binding of Yersinia antigens and immunoglobulins was a prerequisite for the detection of ICs. The method will be valuable for research into the immunopathogenetic mechanisms leading to reactive arthritis after Yersinia infection.
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