A1 Refereed original research article in a scientific journal
Intranasal administration of chlamydial outer protein N (CopN) induces protection against pulmonary Chlamydia pneumoniae infection in a mouse model
Authors: Tammiruusu A, Penttila T, Lahesmaa R, Sarvas M, Puolakkainen M, Vuola JM
Publisher: ELSEVIER SCI LTD
Publication year: 2007
Journal: Vaccine
Journal name in source: VACCINE
Journal acronym: VACCINE
Volume: 25
Issue: 2
First page : 283
Last page: 290
Number of pages: 8
ISSN: 0264-410X
DOI: https://doi.org/10.1016/j.vaccine.2006.07.043
Abstract
Chlamydia pneunioniae is an intracellular pathogen that grows inside a vacuole, referred to as an inclusion. C. pnetanoniae possess a type III secretion system (TTSS), which allows them to secrete effector molecules into the inclusion membrane and to the host cell cytosol. Proteins such as chlamyclial outer protein N (CopN) that associate with the inclusion membrane are potential targets for the host's MHC-dependent antigen presentation. thereby representing ideal antigen candidates for T cell-based vaccination. The results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. detected as antigen-specific antibody production, lymphocyte proliferation and IFN-gamma production. Furthermore, the immunization induced statistically significant protection against intranasal C. pneunioniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation. Both heat-aggregation of the antigen and the presence of LT adjuvant were required for maximal protective effect. (c) 2006 Elsevier Ltd. All rights reserved.
Chlamydia pneunioniae is an intracellular pathogen that grows inside a vacuole, referred to as an inclusion. C. pnetanoniae possess a type III secretion system (TTSS), which allows them to secrete effector molecules into the inclusion membrane and to the host cell cytosol. Proteins such as chlamyclial outer protein N (CopN) that associate with the inclusion membrane are potential targets for the host's MHC-dependent antigen presentation. thereby representing ideal antigen candidates for T cell-based vaccination. The results of this study showed that intranasal immunization of BALB/c mice with heat-aggregated CopN protein and an Escherichia coli heat-labile toxin (LT) induced a strong immune response. detected as antigen-specific antibody production, lymphocyte proliferation and IFN-gamma production. Furthermore, the immunization induced statistically significant protection against intranasal C. pneunioniae challenge, the level of which correlated with the magnitude of CopN-specific lymphocyte proliferation. Both heat-aggregation of the antigen and the presence of LT adjuvant were required for maximal protective effect. (c) 2006 Elsevier Ltd. All rights reserved.
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