A CONVENIENT METHOD FOR REGIONAL MONOAMINE OXIDASE-A DETERMINATION BY [C-14] CLORGYLINE AUTORADIOGRAPHY

: MURAKAMI M, KONDOH Y, YIN WM, MIZUSAWA S, NAKAMICHI H, TAKAHASHI K, SASAKI H, IIDA H, MIURA S, KANNO I, UEMURA K

Publisher: PERGAMON-ELSEVIER SCIENCE LTD

: 1992

: International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology

: NUCLEAR MEDICINE AND BIOLOGY

: NUCL MED BIOL

: 19

: 6

: 619

: 626

: 8

: 0883-2897

The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [C-14]clorgyline in rat. [C-14]Clorgyline was synthesized by the methylation reaction of N-desmethyl-clorgyline and [C-14]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.