A1 Refereed original research article in a scientific journal
A CONVENIENT METHOD FOR REGIONAL MONOAMINE OXIDASE-A DETERMINATION BY [C-14] CLORGYLINE AUTORADIOGRAPHY
Authors: MURAKAMI M, KONDOH Y, YIN WM, MIZUSAWA S, NAKAMICHI H, TAKAHASHI K, SASAKI H, IIDA H, MIURA S, KANNO I, UEMURA K
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 1992
Journal: International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
Journal name in source: NUCLEAR MEDICINE AND BIOLOGY
Journal acronym: NUCL MED BIOL
Volume: 19
Issue: 6
First page : 619
Last page: 626
Number of pages: 8
ISSN: 0883-2897
Abstract
The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [C-14]clorgyline in rat. [C-14]Clorgyline was synthesized by the methylation reaction of N-desmethyl-clorgyline and [C-14]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.
The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [C-14]clorgyline in rat. [C-14]Clorgyline was synthesized by the methylation reaction of N-desmethyl-clorgyline and [C-14]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.
UTU Research Portal