A1 Refereed original research article in a scientific journal
A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node
Authors: Takeuchi A, Ozawa M, Kanda Y, Kozai M, Ohigashi I, Kurosawa Y, Rahman MA, Kawamura T, Shichida Y, Umemoto E, Miyasaka M, Ludewig B, Takahama Y, Nagasawa T, Katakai T
Publisher: FRONTIERS MEDIA SA
Publication year: 2018
Journal: Frontiers in Immunology
Journal name in source: FRONTIERS IN IMMUNOLOGY
Journal acronym: FRONT IMMUNOL
Article number: ARTN 2196
Volume: 9
Number of pages: 17
ISSN: 1664-3224
DOI: https://doi.org/10.3389/fimmu.2018.02196
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/36032927
The spatiotemporal regulation of immune responses in the lymph node (LN) depends on its sophisticated tissue architecture, consisting of several subcompartments supported by distinct fibroblastic stromal cells (FSCs). However, the intricate details of stomal structures and associated FSC subsets are not fully understood. Using several gene reporter mice, we sought to discover unrecognized stromal structures and FSCs in the LN. The four previously identified FSC subsets in the cortex are clearly distinguished by the expression pattern of reporters including PDGFR beta, CCL21-ser, and CXCL12. Herein, we identified a unique FSC subset expressing both CCL21-ser and CXCL12 in the deep cortex periphery (DCP) that is characterized by preferential B cell localization. This subset was clearly different from CXCL12(high) LepR(high) FSCs in the medullary cord, which harbors plasma cells. B cell localization in the DCP was controlled chiefly by CCL21-ser and, to a lesser extent, CXCL12. Moreover, the optimal development of the DCP as well as medulla requires B cells. Together, our findings suggest the presence of a unique microenvironment in the cortex-medulla boundary and offer an advanced view of the multi-layered stromal framework constructed by distinct FSC subsets in the LN.
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