A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction
Tekijät: Ilkka Paatero, Loïc Sauteur, Minkyoung Lee, Anne K. Lagendijk, Daniel Heutschi, Cora Wiesner, Camilo Guzmán, Dimitri Bieli, Benjamin M. Hogan, Markus Affolter, Heinz-Georg Belting
Kustantaja: NATURE PUBLISHING GROUP
Julkaisuvuosi: 2018
Journal: Nature Communications
Tietokannassa oleva lehden nimi: NATURE COMMUNICATIONS
Lehden akronyymi: NAT COMMUN
Artikkelin numero: 3545
Vuosikerta: 9
Aloitussivu: 1
Lopetussivu: 13
Sivujen määrä: 13
ISSN: 2041-1723
DOI: https://doi.org/10.1038/s41467-018-05851-9
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/35817952
Angiogenesis and vascular remodeling are driven by extensive endothelial cell movements. Here, we present in vivo evidence that endothelial cell movements are associated with oscillating lamellipodia-like structures, which emerge from cell junctions in the direction of cell movements. High-resolution time-lapse imaging of these junction-based lamellipodia (JBL) shows dynamic and distinct deployment of junctional proteins, such as F-actin, VE-cadherin and ZO1, during JBL oscillations. Upon initiation, F-actin and VE-cadherin are broadly distributed within JBL, whereas ZO1 remains at cell junctions. Subsequently, a new junction is formed at the front of the JBL, which then merges with the proximal junction. Rac1 inhibition interferes with JBL oscillations and disrupts cell elongation-similar to a truncation in ve-cadherin preventing VE-cad/F-actin interaction. Taken together, our observations suggest an oscillating ratchet-like mechanism, which is used by endothelial cells to move over each other and thus provides the physical means for cell rearrangements.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
