Construction and Characterization of Synthetic Bacterial Community for Experimental Ecology and Evolution



: Cairns J, Jokela R, Hultman J, Tamminen M, Virta M, Hiltunen T

Publisher: FRONTIERS MEDIA SA

: 2018

: Frontiers in Genetics

: FRONTIERS IN GENETICS

: FRONT GENET

: ARTN 312

: 9

: 12

: 1664-8021

DOI: https://doi.org/10.3389/fgene.2018.00312

: https://research.utu.fi/converis/portal/detail/Publication/35726847


Experimental microbial ecology and evolution have yielded foundational insights into ecological and evolutionary processes using simple microcosm setups and phenotypic assays with one- or two-species model systems. The fields are now increasingly incorporating more complex systems and exploration of the molecular basis of observations. For this purpose, simplified, manageable and well-defined multispecies model systems are required that can be easily investigated using culturing and high-throughput sequencing approaches, bridging the gap between simpler and more complex synthetic or natural systems. Here we address this need by constructing a completely synthetic 33 bacterial strain community that can be cultured in simple laboratory conditions. We provide whole-genome data for all the strains as well as metadata about genomic features and phenotypic traits that allow resolving individual strains by amplicon sequencing and facilitate a variety of envisioned mechanistic studies. We further show that a large proportion of the strains exhibit coexistence in co-culture over serial transfer for 48 days in the absence of any experimental manipulation to maintain diversity. The constructed bacterial community can be a valuable resource in future experimental work.

Last updated on 2024-26-11 at 14:38