A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
The mechanism of the metal ion promoted cleavage of RNA phosphodiester bonds involves a general acid catalysis by the metal aquo ion on the departure of the leaving group
Tekijät: Mikkola S, Stenman E, Nurmi K, Yousefi-Salakdeh E, Stromberg R, Lonnberg H
Kustantaja: ROYAL SOC CHEMISTRY
Julkaisuvuosi: 1999
Journal: Royal Society of Chemistry. Journal: Perkin Transactions 2
Tietokannassa oleva lehden nimi: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
Lehden akronyymi: J CHEM SOC PERK T 2
Numero: 8
Aloitussivu: 1619
Lopetussivu: 1625
Sivujen määrä: 7
ISSN: 0300-9580
DOI: https://doi.org/10.1039/a903691a
Tiivistelmä
A series of uridine 3'-alkyl phosphates and 3'-aryl phosphates were synthesised and their cleavage was studied in the presence of Zn2+ aquo ions. A beta(lg) value was determined for the Zn2+ promoted cleavage of both types of compounds. Comparison of the results obtained to those reported previously for the cleavage of the same substrates in the absence of metal ion catalysts suggests that the alkyl leaving group departs as an alcohol in the presence of metal ion catalysts. Furthermore, metal ion catalysts seem to enhance the departure. The aryl leaving group, in contrast, departs as an oxyanion.
A series of uridine 3'-alkyl phosphates and 3'-aryl phosphates were synthesised and their cleavage was studied in the presence of Zn2+ aquo ions. A beta(lg) value was determined for the Zn2+ promoted cleavage of both types of compounds. Comparison of the results obtained to those reported previously for the cleavage of the same substrates in the absence of metal ion catalysts suggests that the alkyl leaving group departs as an alcohol in the presence of metal ion catalysts. Furthermore, metal ion catalysts seem to enhance the departure. The aryl leaving group, in contrast, departs as an oxyanion.
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