A1 Refereed original research article in a scientific journal

The mechanism of the metal ion promoted cleavage of RNA phosphodiester bonds involves a general acid catalysis by the metal aquo ion on the departure of the leaving group




AuthorsMikkola S, Stenman E, Nurmi K, Yousefi-Salakdeh E, Stromberg R, Lonnberg H

PublisherROYAL SOC CHEMISTRY

Publication year1999

JournalRoyal Society of Chemistry. Journal: Perkin Transactions 2

Journal name in sourceJOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2

Journal acronymJ CHEM SOC PERK T 2

Issue8

First page 1619

Last page1625

Number of pages7

ISSN0300-9580

DOIhttps://doi.org/10.1039/a903691a


Abstract
A series of uridine 3'-alkyl phosphates and 3'-aryl phosphates were synthesised and their cleavage was studied in the presence of Zn2+ aquo ions. A beta(lg) value was determined for the Zn2+ promoted cleavage of both types of compounds. Comparison of the results obtained to those reported previously for the cleavage of the same substrates in the absence of metal ion catalysts suggests that the alkyl leaving group departs as an alcohol in the presence of metal ion catalysts. Furthermore, metal ion catalysts seem to enhance the departure. The aryl leaving group, in contrast, departs as an oxyanion.


Research Areas



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