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Oligonucleotides bearing pentaerythritol-derived mass tags
Tekijät: Kiviniemi A, Heinonen P, Lonnberg H
Kustantaja: VERSITA
Julkaisuvuosi: 2007
Lehti:: Central European Journal of Chemistry
Tietokannassa oleva lehden nimi: CENTRAL EUROPEAN JOURNAL OF CHEMISTRY
Lehden akronyymi: CENT EUR J CHEM
Vuosikerta: 5
Numero: 1
Aloitussivu: 191
Lopetussivu: 200
Sivujen määrä: 10
ISSN: 1895-1066
DOI: https://doi.org/10.2478/s11532-006-0046-9
Tiivistelmä
Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a-e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a-f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements. (C) Versita Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.
Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a-e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a-f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements. (C) Versita Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.
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