A1 Refereed original research article in a scientific journal
Oligonucleotides bearing pentaerythritol-derived mass tags
Authors: Kiviniemi A, Heinonen P, Lonnberg H
Publisher: VERSITA
Publication year: 2007
Journal:: Central European Journal of Chemistry
Journal name in source: CENTRAL EUROPEAN JOURNAL OF CHEMISTRY
Journal acronym: CENT EUR J CHEM
Volume: 5
Issue: 1
First page : 191
Last page: 200
Number of pages: 10
ISSN: 1895-1066
DOI: https://doi.org/10.2478/s11532-006-0046-9
Abstract
Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a-e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a-f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements. (C) Versita Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.
Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a-e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a-f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements. (C) Versita Warsaw and Springer-Verlag Berlin Heidelberg. All rights reserved.
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