A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Elevated levels of 8-OHdG and PARK7/DJ-1 in peri-implantitis mucosa
Tekijät: Kasnak G, Firatli E, Kononen E, Olgac V, Zeidan-Chulia F, Gursoy UK
Kustantaja: WILEY
Julkaisuvuosi: 2018
Journal: Clinical Implant Dentistry and Related Research
Tietokannassa oleva lehden nimi: CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH
Lehden akronyymi: CLIN IMPLANT DENT R
Vuosikerta: 20
Numero: 4
Aloitussivu: 574
Lopetussivu: 582
Sivujen määrä: 9
ISSN: 1523-0899
DOI: https://doi.org/10.1111/cid.12619
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/35475695
BackgroundReactive oxygen species contribute to periodontal tissue homeostasis under control of anti-oxidative responses. Disruption in this balance induces severe inflammation and extended tissue degradation.PurposeAim of this study was to identify the expression levels of nuclear factor, erythroid 2 like 2 (NFE2L2/NRF2), Parkinsonism associated deglycase (PARK7/DJ-1), kelch-like ECH associated protein 1 (KEAP1), and 8-hydroxy-deoxyguanosine (8-OHdG) in peri-implant mucosal tissues affected by peri-implantitis, and to compare the levels to those of periodontally diseased and healthy tissue samples.MethodsTissue biopsies were collected from systemically healthy, non-smoking 12 peri-implantitis patients, 13 periodontitis patients, and 13 periodontally healthy controls. Expression levels of NFE2L2/NRF2, PARK7/DJ-1, KEAP1, and 8-OHdG in tissue samples were analyzed immunohistochemically. Statistical analysis was performed by one-way ANOVA with Tukey's HSD test.ResultsInflammatory cell infiltration in the connective tissue and loss of architecture in the spinous layer of the epithelium were prominent in peri-implantitis. Proportions of 8-OHdG and PARK7/DJ-1 expressing cells were elevated in both peri-implantitis (P = .025 for 8-OHdG and P = .014 for PARK7/DJ-1) and periodontitis (P = .038 for 8-OHdG and P = .012 for PARK7/DJ-1) groups in comparison with controls. Staining intensities of 8-OHdG and PARK7/DJ-1 were higher in the periodontitis and peri-implantitis groups than in the control (P < .01) groups. There was no difference in the expression levels of NFE2L2/NRF2 between the groups. KEAP1 was not observed in any tissue sample.ConclusionsPeri-implantitis is characterized by severe inflammation and architectural changes in the epithelium and connective tissue. The expressions of 8-OHdG and PARK7/DJ-1 are elevated in both peri-implantitis and periodontitis.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
