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Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species




TekijätSchwochow D, Serieys LEK, Wayne RK, Thalmann O

KustantajaBIOMED CENTRAL LTD

Julkaisuvuosi2012

Lehti:BMC Biotechnology

Tietokannassa oleva lehden nimiBMC BIOTECHNOLOGY

Lehden akronyymiBMC BIOTECHNOL

Artikkelin numeroARTN 33

Vuosikerta12

Aloitussivu33

Sivujen määrä12

ISSN1472-6750

DOIhttps://doi.org/10.1186/1472-6750-12-33


Tiivistelmä

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.




Last updated on 2024-26-11 at 12:20