Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species - UTU Research Portal

A1 Refereed original research article in a scientific journal

Efficient recovery of whole blood RNA - a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife species

Authors: Schwochow D, Serieys LEK, Wayne RK, Thalmann O

Publisher: BIOMED CENTRAL LTD

Publication year: 2012

Journal: BMC Biotechnology

Journal name in source: BMC BIOTECHNOLOGY

Journal acronym: BMC BIOTECHNOL

Article number: ARTN 33

Volume: 12

First page : 33

Number of pages: 12

ISSN: 1472-6750

DOI: https://doi.org/10.1186/1472-6750-12-33

Abstract

Conclusion: By carefully choosing the appropriate RNA extraction method, whole blood can become a valuable source for high-throughput applications like expression arrays or transcriptome sequencing from natural populations. Additionally, candidate genes showing signs of selection could subsequently be genotyped in large population samples using whole blood as a source for RNA without harming individuals from rare or endangered species.