Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen



: Sergey A. Khorobrykh, Maarit Karonen, Esa Tyystjärvi

: 2015

: FEBS Letters

: 589

: 6

: 779

: 786

: 8

: 0014-5793

DOI: https://doi.org/10.1016/j.febslet.2015.02.011



Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated

PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet

oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was

found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the

same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9

may be an important source of H2O2 within the lipophilic thylakoid membrane.



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