A1 Refereed original research article in a scientific journal
Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen
Authors: Sergey A. Khorobrykh, Maarit Karonen, Esa Tyystjärvi
Publication year: 2015
Journal: FEBS Letters
Volume: 589
Issue: 6
First page : 779
Last page: 786
Number of pages: 8
ISSN: 0014-5793
DOI: https://doi.org/10.1016/j.febslet.2015.02.011
Abstract
Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated
PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet
oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was
found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the
same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9
may be an important source of H2O2 within the lipophilic thylakoid membrane.
Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated
PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet
oxygen (1O2) and analyzed the products. The main reaction of 1O2 with PQH2-9 in methanol was
found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the
same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between 1O2 and PQH2-9
may be an important source of H2O2 within the lipophilic thylakoid membrane.
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