A1 Refereed original research article in a scientific journal
Intra- and intermolecular interactions influence the reactivity of RNA oligonucleotides
Authors: Linjalahti H, Mikkola S
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2007
Journal: Chemistry and Biodiversity
Journal name in source: CHEMISTRY & BIODIVERSITY
Journal acronym: CHEM BIODIVERS
Volume: 4
Issue: 12
First page : 2938
Last page: 2947
Number of pages: 10
ISSN: 1612-1872
DOI: https://doi.org/10.1002/cbdv.200790243(external)
The transesterification of RNA oligonucleotides was studied over a wide pH range. The rate constants obtained indicate that, under neutral conditions, oligonucleotides with an adenosine moiety as the 5'-linked nucleoside can be up to 1000-fold more reactive than the reference oligonucleotide, a 13-mer oligo-U (1). Experiments with the modified oligonucleotide with N-6,N-6-dimethyladenosine (9) as the 5'-Iinked nucleoside moiety suggest that the exocyclic amino group is involved in the reaction, influencing the reactivity of the neighboring phosphodiester bond. In addition to such intramolecular interactions, weak intermolecular interactions most probably contribute to the reactivity.