A1 Refereed original research article in a scientific journal

Intra- and intermolecular interactions influence the reactivity of RNA oligonucleotides




AuthorsLinjalahti H, Mikkola S

PublisherWILEY-V C H VERLAG GMBH

Publication year2007

JournalChemistry and Biodiversity

Journal name in sourceCHEMISTRY & BIODIVERSITY

Journal acronymCHEM BIODIVERS

Volume4

Issue12

First page 2938

Last page2947

Number of pages10

ISSN1612-1872

DOIhttps://doi.org/10.1002/cbdv.200790243(external)


Abstract

The transesterification of RNA oligonucleotides was studied over a wide pH range. The rate constants obtained indicate that, under neutral conditions, oligonucleotides with an adenosine moiety as the 5'-linked nucleoside can be up to 1000-fold more reactive than the reference oligonucleotide, a 13-mer oligo-U (1). Experiments with the modified oligonucleotide with N-6,N-6-dimethyladenosine (9) as the 5'-Iinked nucleoside moiety suggest that the exocyclic amino group is involved in the reaction, influencing the reactivity of the neighboring phosphodiester bond. In addition to such intramolecular interactions, weak intermolecular interactions most probably contribute to the reactivity.



Last updated on 2024-26-11 at 22:20