A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out



: Robert J. Meier, Johann M. B. Simbürger, Tero Soukka, Michael Schäferling

Publisher: ROYAL SOC CHEMISTRY

: 2015

: Chemical Communications

: CHEMICAL COMMUNICATIONS

: CHEM COMMUN

: 51

: 28

: 6145

: 6148

: 4

: 1359-7345

DOI: https://doi.org/10.1039/c5cc00144g


A luminescent probe for determination of pH was designed based on a Forster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.




Last updated on 2024-26-11 at 21:17