A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out - UTU Research Portal

A1 Refereed original research article in a scientific journal

A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

Authors: Robert J. Meier, Johann M. B. Simbürger, Tero Soukka, Michael Schäferling

Publisher: ROYAL SOC CHEMISTRY

Publication year: 2015

Journal: Chemical Communications

Journal name in source: CHEMICAL COMMUNICATIONS

Journal acronym: CHEM COMMUN

Volume: 51

Issue: 28

First page : 6145

Last page: 6148

Number of pages: 4

ISSN: 1359-7345

DOI: https://doi.org/10.1039/c5cc00144g(external)

Abstract

A luminescent probe for determination of pH was designed based on a Forster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.