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Palladacyclic Conjugate Group Promotes Hybridization of Short Oligonucleotides




TekijätMadhuri Hande, Sajal Maity, Tuomas Lönnberg

KustantajaMDPI

Julkaisuvuosi2018

JournalInternational Journal of Molecular Sciences

Tietokannassa oleva lehden nimiINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Lehden akronyymiINT J MOL SCI

Artikkelin numeroARTN 1588

Vuosikerta19

Numero6

Sivujen määrä11

ISSN1422-0067

eISSN1422-0067

DOIhttps://doi.org/10.3390/ijms19061588

Rinnakkaistallenteen osoitehttps://research.utu.fi/converis/portal/detail/Publication/32794093


Tiivistelmä
Short oligonucleotides with cyclopalladated benzylamine moieties at their 5-termini have been prepared to test the possibility of conferring palladacyclic anticancer agents sequence-selectivity by conjugation with a guiding oligonucleotide. Hybridization of these oligonucleotides with natural counterparts was studied by UV and CD (circular dichroism) melting experiments in the absence and presence of a competing ligand (2-mercaptoethanol). Cyclopalladated benzylamine proved to be strongly stabilizing relative to unmetalated benzylamine and modestly stabilizing relative to an extra A center dot T base pair. The stabilization was largely abolished in the presence of 2-mercaptoethanol, suggesting direct coordination of Pd(II) to a nucleobase of the complementary strand. In all cases, fidelity of Watson-Crick base pairing between the two strands was retained. Hybridization of the cyclopalladated oligonucleotides was characterized by relatively large negative enthalpy and entropy, consistent with stabilizing Pd(II) coordination partially offset by the entropic penalty of imposing conformational constraints on the flexible diethylene glycol linker between the oligonucleotide and the palladacyclic moiety.

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Last updated on 2024-26-11 at 22:06