A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Loss of ISWI Function in Drosophila Nuclear Bodies Drives Cytoplasmic Redistribution of Drosophila TDP-43
Tekijät: Lo Piccolo L, Bonaccorso R, Attardi A, Li Greci L, Romano G, Sollazzo M, Giurato G, Ingrassia AMR, Feiguin F, Corona DFV, Onorati MC
Kustantaja: MDPI
Julkaisuvuosi: 2018
Lehti:International Journal of Molecular Sciences
Tietokannassa oleva lehden nimiINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lehden akronyymi: INT J MOL SCI
Artikkelin numero: ARTN 1082
Vuosikerta: 19
Numero: 4
Sivujen määrä: 15
ISSN: 1422-0067
DOI: https://doi.org/10.3390/ijms19041082
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/32521124
Over the past decade, evidence has identified a link between protein aggregation, RNA biology, and a subset of degenerative diseases. An important feature of these disorders is the cytoplasmic or nuclear aggregation of RNA-binding proteins (RBPs). Redistribution of RBPs, such as the human TAR DNA-binding 43 protein (TDP-43) from the nucleus to cytoplasmic inclusions is a pathological feature of several diseases. Indeed, sporadic and familial forms of amyotrophic lateral sclerosis (ALS) and fronto-temporal lobar degeneration share as hallmarks ubiquitin-positive inclusions. Recently, the wide spectrum of neurodegenerative diseases characterized by RBPs functions' alteration and loss was collectively named proteinopathies. Here, we show that TBPH (TAR DNA-binding protein-43 homolog), the Drosophila ortholog of human TDP-43 TAR DNA-binding protein-43, interacts with the arcRNA hsr omega and with hsr omega-associated hnRNPs. Additionally, we found that the loss of the omega speckles remodeler ISWI (Imitation SWI) changes the TBPH sub-cellular localization to drive a TBPH cytoplasmic accumulation. Our results, hence, identify TBPH as a new component of omega speckles and highlight a role of chromatin remodelers in hnRNPs nuclear compartmentalization.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
