A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Tonsillar cytokine expression between patients with tonsillar hypertrophy and recurrent tonsillitis
Tekijät: Mikola E, Elenius V, Saarinen M, Palomares O, Waris M, Turunen R, Puhakka T, Ivaska L, Ruckert B, Aab A, Vahlberg T, Vuorinen T, Allander T, Camargo CA, Akdis M, Akdis CA, Jartti T, Jartti T
Kustantaja: BIOMED CENTRAL LTD
Julkaisuvuosi: 2018
Journal: Clinical and Translational Allergy
Tietokannassa oleva lehden nimi: CLINICAL AND TRANSLATIONAL ALLERGY
Lehden akronyymi: CLIN TRANSL ALLERGY
Artikkelin numero: ARTN 22
Vuosikerta: 8
Sivujen määrä: 8
ISSN: 2045-7022
DOI: https://doi.org/10.1186/s13601-018-0205-z
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/32137333
Background: Tonsils provide an innovative in vivo model for investigating immune response to infections and allergens. However, data are scarce on the differences in tonsillar virus infections and immune responses between patients with tonsillar hypertrophy or recurrent tonsillitis. We investigated the differences in virus detection and T cell and interferon gene expression in patients undergoing tonsillectomy due to tonsillar hypertrophy or recurrent tonsillitis.Methods: Tonsils of 89 surgical patients with tonsillar hypertrophy (n = 47) or recurrent tonsillitis (n = 42) were analysed. Patients were carefully characterized clinically. Standard questionnaire was used to asses preceding and allergy symptoms. Respiratory viruses were analysed in tonsils and nasopharynx by PCR. Quantitative real-time PCR was used to analyse intratonsillar gene expressions of IFN-alpha, IFN-beta, IFN-gamma, IL-10, IL-13, IL-17, IL-28, IL-29, IL-37, TGF-beta, FOXP3, GATA3, RORC2 and Tbet.Results: Median age of the subjects was 15 years (range 2-60). Patients with tonsillar hypertrophy were younger, smoked less often, had less pollen allergy and had more adenovirus, bocavirus-1, coronavirus and rhinovirus in nasopharynx (all P < 0.05). Only bocavirus-1 was more often detected in hypertrophic tonsils (P < 0.05). In age-adjusted analysis, tonsillar hypertrophy was associated with higher mRNA expressions of IL-37 (P < 0.05).Conclusions: Intratonsillar T cell and interferon gene expressions appeared to be relatively stable for both tonsillar hypertrophy and recurrent tonsillitis. Of the studied cytokines, only newly discovered anti-inflammatory cytokine IL-37, was independently associated with tonsillar hypertrophy showing slightly stronger anti-inflammatory response in these patients.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
