A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Structural basis for Acinetobacter baumannii biofilm formation
Tekijät: Natalia Pakharukova, Minna Tuittila, Sari Paavilainen, Henri Malmi, Olena Parilova, Susann Teneberg, Stefan D. Knight, Anton V. Zavialov
Kustantaja: NATL ACAD SCIENCES
Julkaisuvuosi: 2018
Journal: Proceedings of the National Academy of Sciences of the United States of America
Tietokannassa oleva lehden nimi: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Lehden akronyymi: P NATL ACAD SCI USA
Vuosikerta: 115
Numero: 21
Aloitussivu: 5558
Lopetussivu: 5563
Sivujen määrä: 6
ISSN: 0027-8424
eISSN: 1091-6490
DOI: https://doi.org/10.1073/pnas.1800961115
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/32020818
Acinetobacter baumannii-a leading cause of nosocomial infections-has a remarkable capacity to persist in hospital environments and medical devices due to its ability to form biofilms. Biofilm formation is mediated by Csu pili, assembled via the "archaic" chaperone-usher pathway. The X-ray structure of the CsuC-CsuE chaperone-adhesin preassembly complex reveals the basis for bacterial attachment to abiotic surfaces. CsuE exposes three hydrophobic finger-like loops at the tip of the pilus. Decreasing the hydrophobicity of these abolishes bacterial attachment, suggesting that archaic pili use tip-fingers to detect and bind to hydrophobic cavities in substrates. Antitip antibody completely blocks biofilm formation, presenting a means to prevent the spread of the pathogen. The use of hydrophilic materials instead of hydrophobic plastics in medical devices may represent another simple and cheap solution to reduce pathogen spread. Phylogenetic analysis suggests that the tip-fingers binding mechanism is shared by all archaic pili carrying two-domain adhesins. The use of flexible fingers instead of classical receptor-binding cavities is presumably more advantageous for attachment to structurally variable substrates, such as abiotic surfaces.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
