Description and DNA barcoding of Tipula (Pterelachisus) recondita sp n. from the Palaearctic region (Diptera, Tipulidae)

: Pilipenko VE, Salmela J, Vesterinen EJ

Publisher: PENSOFT PUBL PENSOFT PUBL

: 2012

: ZooKeys

: ZOOKEYS

: 192

: 51

: 65

: 15

: 1313-2989

DOI: https://doi.org/10.3897/zookeys.192.2364

: http://www.pensoft.net/journals/zookeys/article/2364/

Tipula (Pterelachisus) recondita Pilipenko & Salmela, sp. n. is described. The new species is collected from two localities: Finland, Kittila (North boreal ecoregion) and Russia, Primorski kray (Zone of temperate broadleaf and mixed forests). Although variation in the structure of male hypopygium between the Finnish and Russian populations is observed, DNA barcode sequences differ only by three nucleotides (0.2 % K2P distance), supporting presence of one widespread species. K2P minimum distances between the new species and 17 other species of the subgenus range from 5.3 to 15.8 % (mean 8.8 %). The new species 1: forest-dwelling, known from an old-growth herb-rich forest (Finland) and Quercus mongolica forest (Russia). The new species is perhaps closest to T (P.) imitator Alexander and in lesser extent to T (P.) pauli Mannheims; the inner gonostylus of both species are illustrated.