Nonlinear filtering in improving the image quality of confocal fluorescent images

: Hänninen P., Stelzer E., Salo J.

Publisher: Springer-Verlag

: 1991

Machine Vision and Applications

: Machine Vision and Applications

: 4

: 243

: 253

: 0932-8092

DOI: https://doi.org/10.1007/BF01815301

: http://api.elsevier.com/content/abstract/scopus_id:0026227162

A different way of processing confocally scanned fluorescence images is presented. Linear median hybrid methods and linear filtering methods are compared numerically with a conventionally processed artificial data set and with real confocal data. The use of linear median hybrid techniques reduces the time required for recording three-dimensional data sets with a confocal fluorescence microscope as well as the photo-damage to the biological sample. The implementation of a linear median algorithm on the hardware level of a confocal microscope is discussed. © 1991 Springer-Verlag New York Inc.