A1 Refereed original research article in a scientific journal
Femtosecond pulse broadening in the focal region of a two-photon fluorescence microscope
Authors: Hanninen P., Hell S.
Publication year: 1994
Journal:: Bioimaging
Journal name in source: Bioimaging
Volume: 2
Issue: 3
First page : 117
Last page: 121
Number of pages: 5
ISSN: 0966-9051
DOI: https://doi.org/10.1002/1361-6374(199409)2:3<117::AID-BIO1>3.0.CO;2-9
Web address : http://api.elsevier.com/content/abstract/scopus_id:0028608767
Abstract
We have determined the broadening of 130 fs and 80 fs pulses in the focus of a high numerical aperture microscope. The focal pulse length has been measured by cross-correlating two counter-propagating pulses at the focus of a 4Pi confocal microscope. This method allowed us to determine the pulse length directly in the sample. Focusing through refractive index interfaces leads to pulse broadening depending on the change in refractive index. Our results are relevant to the field of two-photon fluorescence microscopy and studies of nonlinear phenomena with high spatial resolution.
We have determined the broadening of 130 fs and 80 fs pulses in the focus of a high numerical aperture microscope. The focal pulse length has been measured by cross-correlating two counter-propagating pulses at the focus of a 4Pi confocal microscope. This method allowed us to determine the pulse length directly in the sample. Focusing through refractive index interfaces leads to pulse broadening depending on the change in refractive index. Our results are relevant to the field of two-photon fluorescence microscopy and studies of nonlinear phenomena with high spatial resolution.
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