A1 Refereed original research article in a scientific journal
Image formation and data acquisition in a stage scanning 4Pi confocal fluorescence microscope
Authors: Soini J., Schrader M., Hänninen P., Hell S.
Publication year: 1997
Journal:: Applied Optics
Journal name in source: Applied Optics
Volume: 36
Issue: 34
First page : 8929
Last page: 8934
Number of pages: 6
ISSN: 0003-6935
Web address : http://api.elsevier.com/content/abstract/scopus_id:0031342753
Abstract
We describe the three-dimensional (3-D) image formation and data acquisition in a stage scanning 4Pi confocal fluorescence microscope with the use of two-photon excitation. The 3-D point-spread functions of the 4Pi confocal and regular confocal microscope are measured and compared. Particular emphasis is given to the data acquisition procedure. 4Pi confocal microscopy results in a point-spread function that is 4 times sharper than that of a regular confocal microscope, ultimately leading to superior 3-D imaging of translucent fluorescent specimens. For a two-photon excitation wavelength of approximately 800 nm, we obtain an axial resolution of 140 nm. © 1997 Optical Society of America.
We describe the three-dimensional (3-D) image formation and data acquisition in a stage scanning 4Pi confocal fluorescence microscope with the use of two-photon excitation. The 3-D point-spread functions of the 4Pi confocal and regular confocal microscope are measured and compared. Particular emphasis is given to the data acquisition procedure. 4Pi confocal microscopy results in a point-spread function that is 4 times sharper than that of a regular confocal microscope, ultimately leading to superior 3-D imaging of translucent fluorescent specimens. For a two-photon excitation wavelength of approximately 800 nm, we obtain an axial resolution of 140 nm. © 1997 Optical Society of America.
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