C1 Refereed scientific book
Two-photon excitation fluorescence bioassays
Authors: Hänninen P., Soukka J., Soini J.
Publication year: 2008
Journal name in source: Annals of the New York Academy of Sciences
Volume: 1130
Number of pages: 7
ISBN: 978-1-57331-716-0
ISSN: 0077-8923
DOI: https://doi.org/10.1196/annals.1430.040
Web address : http://api.elsevier.com/content/abstract/scopus_id:47249125734
Abstract
Application of two-photon excitation of fluorescence in microscopy is one of the major discoveries of the "renaissance" of light microscopy that started in the 1980s. The technique derives its advantages from the biologically "smooth" wavelength of the excitation light and the confinement of the excitation. Difficult, and seemingly nontransparent, samples may be imaged with the technique with good resolution. Although the bioresearch has been concentrating mostly on the positive properties of the technique for imaging, the same properties may be applied successfully to nonimaging bioassays. This article focuses on the development path of two-photon excitation-based assay system. © 2008 New York Academy of Sciences.
Application of two-photon excitation of fluorescence in microscopy is one of the major discoveries of the "renaissance" of light microscopy that started in the 1980s. The technique derives its advantages from the biologically "smooth" wavelength of the excitation light and the confinement of the excitation. Difficult, and seemingly nontransparent, samples may be imaged with the technique with good resolution. Although the bioresearch has been concentrating mostly on the positive properties of the technique for imaging, the same properties may be applied successfully to nonimaging bioassays. This article focuses on the development path of two-photon excitation-based assay system. © 2008 New York Academy of Sciences.
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