A1 Refereed original research article in a scientific journal

Continuous wave excitation two-photon fluorescence microscopy



Authors: Hanninen P., Soini E., Hell S.

Publication year: 1994

Journal:: Journal of Microscopy

Journal name in source: Journal of Microscopy

Volume: 176

Issue: 3

First page : 222

Last page: 225

ISSN: 0022-2720

Web address : http://api.elsevier.com/content/abstract/scopus_id:0028579729


Abstract
Two-photon excitation fluorescence imaging is feasible with continuous wave lasers. Images of biological specimens are obtained by employing photon counting in conjunction with an increased recording time. The approach allows two-photon three-dimensional imaging of fluorescently labelled specimens with inexpensive lasers.



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