A1 Refereed original research article in a scientific journal
A new design of the flow cuvette and optical set-up for the scanning flow cytometer
Authors: Soini J., Chernyshev A., Hänninen P., Soini E., Maltsev V.
Publication year: 1998
Journal:: Cytometry
Journal name in source: Cytometry
Volume: 31
Issue: 2
First page : 78
Last page: 84
Number of pages: 7
ISSN: 0196-4763
DOI: https://doi.org/10.1002/(SICI)1097-0320(19980201)31:2<78::AID-CYTO2>3.0.CO;2-E
Web address : http://api.elsevier.com/content/abstract/scopus_id:0031889294
Abstract
We introduce a new design for the optical cuvette and a new optical lay- out for the Scanning Flow Cytometer (SFC) that permits measurement of the angular dependency of the scattered light from individual moving particles. The improved optical scheme of the SFC allows measurement of the angular scattering pattern of individual particles at polar angles from 10°to 120°with integration at azimuthal angles from 0°to 360°and with angular resolution of better than 0.5°. The performance of the SFC is demonstrated using certified polystyrene particles as reference material. The aim of this work is to develop a flow cytometer, which, by recording the entire light scattering pattern of individual biological particles, would provide more information about the particle structure than the ordinary wide angle, forward and side scattering concepts.
We introduce a new design for the optical cuvette and a new optical lay- out for the Scanning Flow Cytometer (SFC) that permits measurement of the angular dependency of the scattered light from individual moving particles. The improved optical scheme of the SFC allows measurement of the angular scattering pattern of individual particles at polar angles from 10°to 120°with integration at azimuthal angles from 0°to 360°and with angular resolution of better than 0.5°. The performance of the SFC is demonstrated using certified polystyrene particles as reference material. The aim of this work is to develop a flow cytometer, which, by recording the entire light scattering pattern of individual biological particles, would provide more information about the particle structure than the ordinary wide angle, forward and side scattering concepts.
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