A1 Refereed original research article in a scientific journal
Metabolome Integrated Analysis of High-Temperature Response in Pinus radiata
Authors: Mónica Escandón, Mónica Meijón, Luis Valledor, Jesús Pascual, Gloria Pinto, María Jesús Cañal
Publisher: FRONTIERS MEDIA SA
Publication year: 2018
Journal: Frontiers in Plant Science
Journal name in source: FRONTIERS IN PLANT SCIENCE
Journal acronym: FRONT PLANT SCI
Article number: ARTN 485
Volume: 9
Number of pages: 15
ISSN: 1664-462X
DOI: https://doi.org/10.3389/fpls.2018.00485
Web address : https://www.frontiersin.org/articles/10.3389/fpls.2018.00485/full
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/31095554
The integrative omics approach is crucial to identify the molecular mechanisms underlying high-temperature response in non-model species. Based on future scenarios of heat increase, Pinus radiata plants were exposed to a temperature of 40 degrees C for a period of 5 days, including recovered plants (30 days after last exposure to 40 degrees C) in the analysis. The analysis of the metabolome using complementary mass spectrometry techniques (GC-MS and LC-Orbitrap-MS) allowed the reliable quantification of 2,287 metabolites. The analysis of identified metabolites and highlighter metabolic pathways across heat time exposure reveal the dynamism of the metabolome in relation to high-temperature response in P. radiata, identifying the existence of a turning point (on day 3) at which P. radiata plants changed from an initial stress response program (shorter-term response) to an acclimation one (longer-term response). Furthermore, the integration of metabolome and physiological measurements, which cover from the photosynthetic state to hormonal profile, suggests a complex metabolic pathway interaction network related to heat-stress response. Cytokinins (CKs), fatty acid metabolism and flavonoid and terpenoid biosynthesis were revealed as the most important pathways involved in heat-stress response in P. radiata, with zeatin riboside (ZR) and isopentenyl adenosine (iPA) as the key hormones coordinating these multiple and complex interactions. On the other hand, the integrative approach allowed elucidation of crucial metabolic mechanisms involved in heat response in P. radiata, as well as the identification of thermotolerance metabolic biomarkers (L-phenylalanine, hexadecanoic acid, and dihydromyricetin), crucial metabolites which can reschedule the metabolic strategy to adapt to high temperature.
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