Disruption of a spe-like open reading frame alters polyamine content and psbA-2 mRNA stability in the cyanobacterium Synechocystis sp. PCC 6803



: Mulo P, Eloranta T, Aro EM, Maenpaa P

Publisher: GEORG THIEME VERLAG

: 1998

: Botanica Acta

: BOTANICA ACTA

: BOT ACTA

: 111

: 1

: 71

: 76

: 6

: 0932-8629

DOI: https://doi.org/10.1111/j.1438-8677.1998.tb00680.x


In Synechocystis 6803, transcripts of the psbA-2 gene encoding the reaction-center protein D1 of photosystem II are short-lived in light but stable in darkness. No dark-stabilization was observed if the genomic region downstream from the psbA-2 gene was disrupted. The deleted fragment was found to include an open reading frame (ORF) with striking similarity to the spe gene encoding arginine decarboxylase (L-arginine carboxy-lyase, EC 4.1.1.19), which is involved in the biosynthesis of polyamines. The spermidine content of the mutants with a disrupted ORF was observed to be lower than in the wild-type suggesting that polyamines may play a role in the stability of the psbA-2 mRNA.



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