A1 Refereed original research article in a scientific journal
Disruption of a spe-like open reading frame alters polyamine content and psbA-2 mRNA stability in the cyanobacterium Synechocystis sp. PCC 6803
Authors: Mulo P, Eloranta T, Aro EM, Maenpaa P
Publisher: GEORG THIEME VERLAG
Publication year: 1998
Journal: Botanica Acta
Journal name in source: BOTANICA ACTA
Journal acronym: BOT ACTA
Volume: 111
Issue: 1
First page : 71
Last page: 76
Number of pages: 6
ISSN: 0932-8629
DOI: https://doi.org/10.1111/j.1438-8677.1998.tb00680.x(external)
Abstract
In Synechocystis 6803, transcripts of the psbA-2 gene encoding the reaction-center protein D1 of photosystem II are short-lived in light but stable in darkness. No dark-stabilization was observed if the genomic region downstream from the psbA-2 gene was disrupted. The deleted fragment was found to include an open reading frame (ORF) with striking similarity to the spe gene encoding arginine decarboxylase (L-arginine carboxy-lyase, EC 4.1.1.19), which is involved in the biosynthesis of polyamines. The spermidine content of the mutants with a disrupted ORF was observed to be lower than in the wild-type suggesting that polyamines may play a role in the stability of the psbA-2 mRNA.
In Synechocystis 6803, transcripts of the psbA-2 gene encoding the reaction-center protein D1 of photosystem II are short-lived in light but stable in darkness. No dark-stabilization was observed if the genomic region downstream from the psbA-2 gene was disrupted. The deleted fragment was found to include an open reading frame (ORF) with striking similarity to the spe gene encoding arginine decarboxylase (L-arginine carboxy-lyase, EC 4.1.1.19), which is involved in the biosynthesis of polyamines. The spermidine content of the mutants with a disrupted ORF was observed to be lower than in the wild-type suggesting that polyamines may play a role in the stability of the psbA-2 mRNA.