A1 Refereed original research article in a scientific journal

Disruption of a spe-like open reading frame alters polyamine content and psbA-2 mRNA stability in the cyanobacterium Synechocystis sp. PCC 6803




AuthorsMulo P, Eloranta T, Aro EM, Maenpaa P

PublisherGEORG THIEME VERLAG

Publication year1998

JournalBotanica Acta

Journal name in sourceBOTANICA ACTA

Journal acronymBOT ACTA

Volume111

Issue1

First page 71

Last page76

Number of pages6

ISSN0932-8629

DOIhttps://doi.org/10.1111/j.1438-8677.1998.tb00680.x(external)


Abstract
In Synechocystis 6803, transcripts of the psbA-2 gene encoding the reaction-center protein D1 of photosystem II are short-lived in light but stable in darkness. No dark-stabilization was observed if the genomic region downstream from the psbA-2 gene was disrupted. The deleted fragment was found to include an open reading frame (ORF) with striking similarity to the spe gene encoding arginine decarboxylase (L-arginine carboxy-lyase, EC 4.1.1.19), which is involved in the biosynthesis of polyamines. The spermidine content of the mutants with a disrupted ORF was observed to be lower than in the wild-type suggesting that polyamines may play a role in the stability of the psbA-2 mRNA.



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