New crystal forms of Escherichia coli and Saccharomyces cerevisiae soluble inorganic pyrophosphatases



: HEIKINHEIMO P, SALMINEN T, LAHTI R, COOPERMAN B, GOLDMAN A

Publisher: MUNKSGAARD INT PUBL LTD

: 1995

: Acta Crystallographica Section D: Biological Crystallography

: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

: ACTA CRYSTALLOGR D

: 51

: 399

: 401

: 3

: 0907-4449

DOI: https://doi.org/10.1107/S0907444994010784(external)


We have obtained new crystal forms of Escherichia coil and Saccharomyces cerevisiae soluble inorganic pyrophosphatase with and without substrate, competitive inhibitor and divalent cation. They diffract to higher resolution than any forms previously reported. The best E. coli crystals are in space group R32 with cell dimensions of 111.4 x 111.4 x 76.6 Angstrom and diffract to 2.0 Angstrom. The best S. cerevisiae crystals were grown from a mixture of PEG 1000 and 4000 in the presence of metal ions. They are in space group P2(1)2(1)2(1), have cell dimensions of 54.2 x 68.5 x 161.7 Angstrom and diffract to 1.8 Angstrom.



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