A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
New crystal forms of Escherichia coli and Saccharomyces cerevisiae soluble inorganic pyrophosphatases
Tekijät: HEIKINHEIMO P, SALMINEN T, LAHTI R, COOPERMAN B, GOLDMAN A
Kustantaja: MUNKSGAARD INT PUBL LTD
Julkaisuvuosi: 1995
Journal: Acta Crystallographica Section D: Biological Crystallography
Tietokannassa oleva lehden nimi: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Lehden akronyymi: ACTA CRYSTALLOGR D
Vuosikerta: 51
Aloitussivu: 399
Lopetussivu: 401
Sivujen määrä: 3
ISSN: 0907-4449
DOI: https://doi.org/10.1107/S0907444994010784
Tiivistelmä
We have obtained new crystal forms of Escherichia coil and Saccharomyces cerevisiae soluble inorganic pyrophosphatase with and without substrate, competitive inhibitor and divalent cation. They diffract to higher resolution than any forms previously reported. The best E. coli crystals are in space group R32 with cell dimensions of 111.4 x 111.4 x 76.6 Angstrom and diffract to 2.0 Angstrom. The best S. cerevisiae crystals were grown from a mixture of PEG 1000 and 4000 in the presence of metal ions. They are in space group P2(1)2(1)2(1), have cell dimensions of 54.2 x 68.5 x 161.7 Angstrom and diffract to 1.8 Angstrom.
We have obtained new crystal forms of Escherichia coil and Saccharomyces cerevisiae soluble inorganic pyrophosphatase with and without substrate, competitive inhibitor and divalent cation. They diffract to higher resolution than any forms previously reported. The best E. coli crystals are in space group R32 with cell dimensions of 111.4 x 111.4 x 76.6 Angstrom and diffract to 2.0 Angstrom. The best S. cerevisiae crystals were grown from a mixture of PEG 1000 and 4000 in the presence of metal ions. They are in space group P2(1)2(1)2(1), have cell dimensions of 54.2 x 68.5 x 161.7 Angstrom and diffract to 1.8 Angstrom.
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