A1 Refereed original research article in a scientific journal
Expression of CPPED1 in human trophoblasts is associated with timing of term birth
Authors: Antti M. Haapalainen, Minna K. Karjalainen, Ravindra Daddali, Steffen Ohlmeier, Julia Anttonen, Tomi A. Määttä, Annamari Salminen, Mari Mahlman, Ulrich Bergmann, Kaarin Mäkikallio, Marja Ojaniemi, Mikko Hallman, Mika Rämet
Publisher: WILEY
Publication year: 2018
Journal: Journal of Cellular and Molecular Medicine
Journal name in source: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Journal acronym: J CELL MOL MED
Volume: 22
Issue: 2
First page : 968
Last page: 981
Number of pages: 14
ISSN: 1582-4934
DOI: https://doi.org/10.1111/jcmm.13402
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/29597618
Understanding of timing of human parturition is incomplete. Therefore, we carried out proteomic analyses of full-term placentas from uncomplicated pregnancies to identify protein signatures associated with the onset of spontaneous delivery. We found quantitative associations of 10 proteins with spontaneous term birth, evident either in the basal or in the chorionic plates or in both. Additional 18 proteins were associated according to the location within placenta indicating local variations in protein amounts. Calcineurin-like phosphoesterase domain-containing 1 (CPPED1), a phosphatase previously suggested dephosphorylating AKT1/PKB, was one of the identified proteins. qRT-PCR revealed the mRNA level of CPPED1 was higher in elective caesarean deliveries than in spontaneous births, while immunohistochemistry showed CPPED1 in cytotrophoblasts, syncytiotrophoblasts and extravillous trophoblasts. Noteworthy, phosphorylation status of AKT1 did not differ between placentas from elective caesarean and spontaneous deliveries. Additionally, analyses of samples from infants indicated that single-nucleotide polymorphisms rs11643593 and rs8048866 of CPPED1 were associated with duration of term pregnancy. Finally, post-transcriptional silencing of CPPED1 in cultured HTR8/SVneo cells by siRNAs affected gene expression in pathways associated with inflammation and blood vessel development. We postulate that functions regulated by CPPED1 in trophoblasts at choriodecidual interphase have a role in the induction of term labour, but it may be independent of AKT1.
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