HYDROLYSIS AND ISOMERIZATION OF THE INTERNUCLEOSIDIC PHOSPHODIESTER BONDS OF POLYURIDYLIC ACID - KINETICS AND MECHANISM



: KUUSELA S, LONNBERG H

Publisher: ROYAL SOC CHEMISTRY

: 1994

: Royal Society of Chemistry. Journal: Perkin Transactions 2

: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2

: J CHEM SOC PERK T 2

: 10

: 2109

: 2113

: 5

: 0300-9580

DOI: https://doi.org/10.1039/p29940002109


The pH-rate profiles for the hydrolysis of the 3',5'-phosphodiester bonds of poly(U) and their isomerization to the 2',5' bonds have been determined by following the progress of the reactions by enzymatic digestion with RNAase A and subsequent RP HPLC. The kinetics observed are compared with those of the hydrolysis and interconversion of uridylyl (3'.5')uridine and uridylyl(2',5') uridine.



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