HYDROLYSIS AND ISOMERIZATION OF THE INTERNUCLEOSIDIC PHOSPHODIESTER BONDS OF POLYURIDYLIC ACID - KINETICS AND MECHANISM - UTU Research Portal

A1 Refereed original research article in a scientific journal

HYDROLYSIS AND ISOMERIZATION OF THE INTERNUCLEOSIDIC PHOSPHODIESTER BONDS OF POLYURIDYLIC ACID - KINETICS AND MECHANISM

Authors: KUUSELA S, LONNBERG H

Publisher: ROYAL SOC CHEMISTRY

Publication year: 1994

Journal: Royal Society of Chemistry. Journal: Perkin Transactions 2

Journal name in source: JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2

Journal acronym: J CHEM SOC PERK T 2

Issue: 10

First page : 2109

Last page: 2113

Number of pages: 5

ISSN: 0300-9580

DOI: https://doi.org/10.1039/p29940002109

Abstract

The pH-rate profiles for the hydrolysis of the 3',5'-phosphodiester bonds of poly(U) and their isomerization to the 2',5' bonds have been determined by following the progress of the reactions by enzymatic digestion with RNAase A and subsequent RP HPLC. The kinetics observed are compared with those of the hydrolysis and interconversion of uridylyl (3'.5')uridine and uridylyl(2',5') uridine.