Applications of the bacteriophage Mu in vitro transposition reaction and genome manipulation via electroporation of DNA transposition complexes
: Saija Haapa-Paananen, Harri Savilahti
: Martha R.J. Clokie, Andrew M. Kropinski, Rob Lavigne
Publisher: Humana Press Inc.
: New York
: 2018
: Bacteriophages: Methods and Protocols, Volume 3
Methods in Molecular Biology
: Methods in Molecular Biology
: 1681
: 279
: 286
: 8
: 978-1-4939-7341-5
: 978-1-4939-7343-9
: 1064-3745
DOI: https://doi.org/10.1007/978-1-4939-7343-9_20
The capacity of transposable elements to insert into the genomes has
been harnessed during the past decades to various in vitro and in vivo
applications. This chapter describes in detail the general protocols and
principles applicable for the Mu in vitro transposition reaction as
well as the assembly of DNA transposition complexes that can be
electroporated into bacterial cells to accomplish efficient gene
delivery. These techniques with their modifications potentiate various
gene and genome modification applications, which are discussed briefly
here, and the reader is referred to the original publications for
further details.
