A3 Vertaisarvioitu kirjan tai muun kokoomateoksen osa
Applications of the bacteriophage Mu in vitro transposition reaction and genome manipulation via electroporation of DNA transposition complexes
Tekijät: Saija Haapa-Paananen, Harri Savilahti
Toimittaja: Martha R.J. Clokie, Andrew M. Kropinski, Rob Lavigne
Kustantaja: Humana Press Inc.
Kustannuspaikka: New York
Julkaisuvuosi: 2018
Kokoomateoksen nimi: Bacteriophages: Methods and Protocols, Volume 3
Tietokannassa oleva lehden nimi: Methods in Molecular Biology
Sarjan nimi: Methods in Molecular Biology
Vuosikerta: 1681
Aloitussivu: 279
Lopetussivu: 286
Sivujen määrä: 8
ISBN: 978-1-4939-7341-5
eISBN: 978-1-4939-7343-9
ISSN: 1064-3745
DOI: https://doi.org/10.1007/978-1-4939-7343-9_20
The capacity of transposable elements to insert into the genomes has
been harnessed during the past decades to various in vitro and in vivo
applications. This chapter describes in detail the general protocols and
principles applicable for the Mu in vitro transposition reaction as
well as the assembly of DNA transposition complexes that can be
electroporated into bacterial cells to accomplish efficient gene
delivery. These techniques with their modifications potentiate various
gene and genome modification applications, which are discussed briefly
here, and the reader is referred to the original publications for
further details.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
