Hydrolytic stability of a phosphate-branched oligonucleotide incorporating a ribonucleoside 3 '-phosphotriester unit

: Lonnberg T

Publisher: TAYLOR & FRANCIS INC

: 2006

Nucleosides, Nucleotides and Nucleic Acids

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

: NUCLEOS NUCLEOT NUCL

: 25

: 3

: 315

: 323

: 9

: 1525-7770

DOI: https://doi.org/10.1080/15257770500544537

A phosphate branched oligonucleotide has been prepared by using an appropriately protected trinucleoside phosphotriester building block in conventional solid-phase synthesis. Hydrolysis of the branched oligonucleotide has been followed over a wide pH range. Comparison of the present results with those previously obtained for simpler analogues indicates that a trinucleoside 3',3',5'-monophosphate, when embedded in an oligonucleotide structure, is stabilized toward hydroxide-ion catalyzed cleavage by more than one order of magnitude, lending some support to the feasibility of existence of phosphate-branched RNA X in biological systems.

Chemical sciences