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Hydrolytic stability of a phosphate-branched oligonucleotide incorporating a ribonucleoside 3 '-phosphotriester unit
Tekijät: Lonnberg T
Kustantaja: TAYLOR & FRANCIS INC
Julkaisuvuosi: 2006
Lehti:Nucleosides, Nucleotides and Nucleic Acids
Tietokannassa oleva lehden nimiNUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Lehden akronyymi: NUCLEOS NUCLEOT NUCL
Vuosikerta: 25
Numero: 3
Aloitussivu: 315
Lopetussivu: 323
Sivujen määrä: 9
ISSN: 1525-7770
DOI: https://doi.org/10.1080/15257770500544537
Tiivistelmä
A phosphate branched oligonucleotide has been prepared by using an appropriately protected trinucleoside phosphotriester building block in conventional solid-phase synthesis. Hydrolysis of the branched oligonucleotide has been followed over a wide pH range. Comparison of the present results with those previously obtained for simpler analogues indicates that a trinucleoside 3',3',5'-monophosphate, when embedded in an oligonucleotide structure, is stabilized toward hydroxide-ion catalyzed cleavage by more than one order of magnitude, lending some support to the feasibility of existence of phosphate-branched RNA X in biological systems.
A phosphate branched oligonucleotide has been prepared by using an appropriately protected trinucleoside phosphotriester building block in conventional solid-phase synthesis. Hydrolysis of the branched oligonucleotide has been followed over a wide pH range. Comparison of the present results with those previously obtained for simpler analogues indicates that a trinucleoside 3',3',5'-monophosphate, when embedded in an oligonucleotide structure, is stabilized toward hydroxide-ion catalyzed cleavage by more than one order of magnitude, lending some support to the feasibility of existence of phosphate-branched RNA X in biological systems.
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