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ASSOCIATION OF NUCLEOSIDES AND THEIR 5'-MONOPHOSPHATES WITH A TRYPTOPHAN CONTAINING TRIPEPTIDE, TRP-LEU-GLU - THE SOURCE OF AN OVERESTIMATION BY FLUORESCENCE SPECTROSCOPY
Tekijät: WIECZOREK Z, STEPINSKI J, DARZYNKIEWICZ E, LONNBERG H
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 1993
Lehti: Biophysical Chemistry
Tietokannassa oleva lehden nimi: BIOPHYSICAL CHEMISTRY
Lehden akronyymi: BIOPHYS CHEM
Vuosikerta: 47
Numero: 3
Aloitussivu: 233
Lopetussivu: 240
Sivujen määrä: 8
ISSN: 0301-4622
DOI: https://doi.org/10.1016/0301-4622(93)80048-N
Tiivistelmä
Association of 7-methylguanosine 5'-monophosphate with a tryptophan containing tripeptide, Trp-Leu-Glu, has been studied by fluorescence titration using two different geometries of detection, viz. right angle and front surface geometry. The applicability of these two techniques to determine the stability constant of the nucleotide-peptide adduct is discussed. Evidence is presented that fluorescence titration based on right angle detection may lead to considerable overestimation of the strength of interaction.
Association of 7-methylguanosine 5'-monophosphate with a tryptophan containing tripeptide, Trp-Leu-Glu, has been studied by fluorescence titration using two different geometries of detection, viz. right angle and front surface geometry. The applicability of these two techniques to determine the stability constant of the nucleotide-peptide adduct is discussed. Evidence is presented that fluorescence titration based on right angle detection may lead to considerable overestimation of the strength of interaction.
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